The Diffusion Of Glucose And Iki: An Experiment Report
Table of Contents
Lab Write Up A
Discussion:
Lab Write Up B
Lab Write Up C
In conclusion
Lab Write Up A
Diffusion:
Aim/Question. If the net movements of molecules are from higher concentrations to lower concentrations in a plasma membrane will the dialysis system turn blue as a result of the diffusion IKI and glucose into the bag.
Hypothesis: I believe that if I mix the glucose with IKI in the distilled waters, it will cause the dye to turn blue. The lab’s objective is to study and understand the semipermeable skin and how molecules diffuse from high concentration to low. The goal of the lab is to test whether the IKI dissolves in the glucose/starch solution by adding it to the water.
Materials: Dialysis tubing.
Procedure:
The cup and the dialysis bag are two options. Two solutions are tested: IKI or glucose/starch solution. To collect data, create a table. In the table, show/describe each initial and final solution colors. The + symbol is for positive results and the – symbol is for negative.
In a cup, pour 160-170ml of distilled water. Next, conduct a Benedict’s glucose test to determine if monosaccharide is present. Add 4 mL IKI solutions to the water. Mix well. Record the initial solution color in the Table 1.
Do the Benedict’s test of monosaccharide using glucose/starch solutions. Record the results.
You will need a piece soaked in water to dialysis tubing. To open the tubing, roll it between your thumbs and index fingers. You can tie one end with string, and make a bag.
To release air, smoothen out the top of your dialysis bag using a funnel. The bag’s top should be tied, but allow enough space for expansion. In Table 1, note the initial color for the glucose/starch solutions.
Dip the dialysis bag and the solution into a cup. Be sure to cover the starch/glucose solution portion.
Wait for about 30 minutes before you begin the next exercise. You will need to draw the experiment (the dialysis bag and cup), and then call it Figure. Indicate on Figure where the molecules are located (insider and outside the bag) before they diffuse through the dialysis membrane. In Figure 2, you will make a prediction about the direction of net diffusion for each of these molecules. Each prediction should be accompanied by a reason.
After 30 minutes, take the bag out of the cup and blot it with paper towel. Cut a small slit in bag big enough to fit a dropper. Finally, fill in Table 1.
A sketch of a laboratory
The figure 2 drawing shows the glucose/starch mixture inside the dialysis bag. Only the glucose can diffuse through the bag. Starch solution is too large to diffuse through the pores of the bag. However, glucose is small enough for the pores to pass. Water molecules and IKI will also easily pass through the pores. The cup will allow glucose to diffuse outwards in order to reach facilitated balance. The water molecules in the cup move by osmosis to more concentrated areas to reach facilitated stability.
Discussion: I was right in my hypothesis that the IKI solution, which contained water, would diffuse into the dialysis bags. My predictions were correct as lab A did not have any conflicts.
Because the starch was not found in the dialysis bag, the data showed that it is larger than the dialysis tubing’s pore size. IKI, glucose solution, and water molecules can also be seen in the data.
Conclusion: Does the string tension affect how much starch or IKI is allowed to diffuse into the bag? I think the string would diffuse both starch and IKI if it were loosely tied. If it was tightly tied, it would not.
Lab Write Up B Osmosis:
Aim/Question – Does the amount of distilled water differ in the solute concentration?
Hypothesis: I add the different concentrations to the solution and hypothesize that this will result in a variation in the amount of solute concentration that is achieved through osmosis.
Objective: To observe how solute concentration diffuses through semipermeable membranes based on the amount of molar concentration that is being used in the lab. It is possible to see the difference by changing the molarity.
Materials: Dialysis tubing.
Procedure:
For each sucrose solution to be tested, follow the below steps.
You will need to pour 160-170 mL distilled water into a plastic container.
Take a piece dialysis tubing and soak it in water. Then, roll it between your thumbs and index fingers. The tubing can be closed by either knotting or stringing one end. This will create a bag.
To expel air, use a small funnel to pour 25mL of sucrose solution in the dialysis bag. You can tie the bag at the end, but allow enough space for expansion.
Dry the bag using paper towels before recording the mass. Table 2: Record the initial mass. Table 2 should have 5 columns (Contents In Dialysis Bag and Initial Mass, Final and % Change in Mass), as well as 6 rows (one per Molar Concentration). You should allow yourself enough space to record all calculations.
The dialysis bag should be immersed in the water. Next, wait for 30 seconds before moving onto the next step.
After 30 seconds, take out the bag from cup and dry on paper towels. In Table 2, weigh the bag. Next, calculate the bag’s mass and record it in Table 2.
Discussion:
The mass change indicates water loss from the dialysis bag. This experiment will measure the percent change on mass. The experiment’s outcome may be affected by two variables. First, there is the amount water inside the dialysis bag. Water molecules in both the permeable membrane and the cup will vary depending on how much. The second variable is the volume of air in dialysis bags. Too much air will prevent water molecules from diffusing into the bag.
The string tied at the top and bottom would also affect how much water has been absorbed. Bag 0.0M was hypertonic. The dialysis bag 0.0M would be hypertonic if the distilled water was added. This is because the water will diffuse from the bag and reach equilibrium. While there were some errors that could have affected the lab’s performance, the result we expected was correct. My results back up my hypothesis. I was able to determine that the mass of each solution varied in concentration.
Although the 0 M setup was meant to be isotonic it proved not to be so. The solution would be hypertonic if the dialysis bag was filled instead with sucrose solution. Seawater can cause dehydration as the cells lose water. Seawater is more concentrated than small intestine cells. To enable cells to reach facilitated balance, water will be lost, which can lead to dehydration.
Conclusion: Does the concentration of a solution affect its mass? The solution’s mass will change depending on how concentrated it is. The bag will gain weight if it has more concentration.
Lab Write up CPlant Cells & Water Potential
Question: How does the sucrose solution change the water potential of potato cylinders?
Hypothesis: It all depends on the solution. Higher concentrations mean more water loss for potatoes. Potatoes have more water potential.
Objective: To understand and observe the water capacity of potato cores, we need to dissolve them in sucrose solution and determine their mass change. We want to know how water diffuses through the tissues of living plants, specifically potatoes.
Materials: Distilled water, sucrose solutions and plastic cups.
Procedure:
Label the cup with the amount of sucrose you are testing.
Cut four cylinders from potato tissue using the cork borer The cork borer should not be used to cut your fingers. To remove skin, cut the potato cylinders to 3 cm in length. Be careful when cutting the ends.
Place the potato tube in a cup.
To determine the total potato mass, use a balance. The initial mass should be recorded on Table 3. You should have seven columns in your table (Contents In Cup, Temperature, Initial Mass, Final Mass, Change in Mass, % Change in Mass and % Change in Class Average) and six rows (one per molarity).
Place the potato portions in the cup. Pour 100mL of your sucrose solution.
Wrap the cup in plastic wrap.
If you are given a second level of sucrose, continue steps 1-6 with this solution.
Stop. Let the potato pieces remain overnight in the sucrose solution. Next, move on to Step 9.
Discussion:
The equilibrium water potentio is 0+0=0. At equilibrium, there is 0. C=mole/Liter. T= 297K. The formula yielded (-1)(0.0831)(297)=-bars. The vegetable slices would gain water because the water potency in its cells is lower. Because there is less water potential, water can move into the slice, allowing it grow and become hypotonic.
I would recommend to the farm manager that he or she stop using the Mediterranean Sea seawater for irrigation. It will not help the wheat but make it dry. Seawater has a lower potential for water than roots. This means that wheat will lose even more water when trying to reach facilitated balance. If a marine snail is accidentally added to freshwater aquariums, its cells will try to reach equilibrium and then become hypotonic. This can lead to the clam dying. The syrup is very concentrated so the carrot cells may lose water. The syrup is very low in potency, so it will not reach the carrot cell’s epidermis. It will absorb all the water from the carrot cells.
Carrot cells are more likely to absorb water than the water so they will be more water-saturated. The syrup absorbs water from carrot cells. This causes them to grow in size, and raises the volume of water in their cups.
ConclusionIs there a difference in the potato cylinders’ water potential due to temperature? I believe the faster molecules diffuse from the bag of dialysis, the more water potential there is.
